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KMID : 0380020140290010058
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Korean Journal of Biotechnology and Bioengineering
2014 Volume.29 No. 1 p.58 ~ p.66
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Quality and Availability Evaluation of Human Hepatocytes Isolated from Resected Partial Livers for Toxicology and Drug Metabolism Studies in Korea
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Noh Jeong-Kwon
Jang In-Keun
Kim Hyo-Eun
Lee Jong-Eun
Yang Mal-Sook
Jang Eun-Mi
Lee Ji-Hyun
Park Hey-Jung
Kim Young-A
Lee Suk-Koo
Jeong Ho-Sang
Ahn Joon-Ik
Lee Doo-Hoon
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Abstract
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Demand for in vitro pharmacological evaluation and toxicity test using human hepatocytes has been increasing. In USA and Europe, human hepatocytes obtained from donated whole liver unsuitable for transplantation were distributed to researchers and deposited in cell bank facility as cryopreserved vial. In Korea, however, incidence of transplantation- inappropriate whole liver has been quite low and the whole livers almost have so severe liver disease such as fatty or fibrotic liver that cannot meet the demand. In this study we aimed to isolate human hepatocytes from liver resection surgery-originated partial liver, and assure the isolated human hepatocytes and its cryopreserved hepatocytes to be qualified for the in vitro pharmacological evaluation and drug toxicity tests. We compared those with commercially available human hepatocyte, BD GenTestTM by cell morphology, hepatic gene expression, urea synthesis, albumin secretion, ammonia removal, and cytochrome P450 induction activities. Changes in hepatotoxic gene expression after cryopreservation are evaluated with a typical hepatotoxic drug, acetaminophen. Consequently, the fresh hepatocytes from the partial liver and its cryopreserved hepatocytes expressed their intrinsic hepatic functions well and showed equal hepatotoxicity gene expression trend regardless to cryopreservation. Therefore, liver resection surgery-originated partial liver can be used as a useful source of human hepatocytes for various pharmacological and hepatotoxicity test.
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KEYWORD
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Human hepatocyte, Partial liver, Drug metabolism, Drug screening
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